Phenotypic and genetic analyses of the heterogeneous population present in the cold-adapted master donor strain: A/Leningrad/134/17/57 (H2N2).
Identifieur interne : 001655 ( Main/Exploration ); précédent : 001654; suivant : 001656Phenotypic and genetic analyses of the heterogeneous population present in the cold-adapted master donor strain: A/Leningrad/134/17/57 (H2N2).
Auteurs : R. Youil [États-Unis] ; I. Kiseleva ; W-S Kwan ; C. Szymkowiak ; T J Toner ; Q. Su ; A. Klimov ; L. Rudenko ; A R ShawSource :
- Virus research [ 0168-1702 ] ; 2004.
Descripteurs français
- KwdFr :
- Adaptation physiologique, Analyse de séquence de protéine, Animaux, Basse température, Cellules Vero, Culture virale, Lignée cellulaire, Mutation faux-sens, Méthode des plages virales, Ovule (virologie), Phénotype, Protéines virales (), Protéines virales (génétique), Protéines virales (physiologie), Sous-type H2N2 du virus de la grippe A, Substitution d'acide aminé, Température, Vaccins antigrippaux, Variation génétique, Virus de la grippe A (croissance et développement), Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification).
- MESH :
- croissance et développement : Virus de la grippe A.
- génétique : Protéines virales, Virus de la grippe A.
- isolement et purification : Virus de la grippe A.
- physiologie : Protéines virales.
- virologie : Ovule.
- Adaptation physiologique, Analyse de séquence de protéine, Animaux, Basse température, Cellules Vero, Culture virale, Lignée cellulaire, Mutation faux-sens, Méthode des plages virales, Phénotype, Protéines virales, Sous-type H2N2 du virus de la grippe A, Substitution d'acide aminé, Température, Vaccins antigrippaux, Variation génétique.
English descriptors
- KwdEn :
- Adaptation, Physiological, Amino Acid Substitution, Animals, Cell Line, Chlorocebus aethiops, Cold Temperature, Genetic Variation, Influenza A Virus, H2N2 Subtype, Influenza A virus (genetics), Influenza A virus (growth & development), Influenza A virus (isolation & purification), Influenza Vaccines, Mutation, Missense, Ovum (virology), Phenotype, Sequence Analysis, Protein, Temperature, Vero Cells, Viral Plaque Assay, Viral Proteins (chemistry), Viral Proteins (genetics), Viral Proteins (physiology), Virus Cultivation.
- MESH :
- chemical , chemistry : Viral Proteins.
- chemical , genetics : Viral Proteins.
- chemical , physiology : Viral Proteins.
- chemical : Influenza Vaccines.
- genetics : Influenza A virus.
- growth & development : Influenza A virus.
- isolation & purification : Influenza A virus.
- virology : Ovum.
- Adaptation, Physiological, Amino Acid Substitution, Animals, Cell Line, Chlorocebus aethiops, Cold Temperature, Genetic Variation, Influenza A Virus, H2N2 Subtype, Mutation, Missense, Phenotype, Sequence Analysis, Protein, Temperature, Vero Cells, Viral Plaque Assay, Virus Cultivation.
Abstract
For the past three decades the cold-adapted (ca) and temperature sensitive (ts) master donor strain, A/Leningrad/134/17/57 (H2N2) has been successfully used as the basis for the live attenuated reassortant influenza A vaccine. This donor strain was developed from A/Leningrad/134/57 (H2N2) wild-type (wt) virus following 17 passages in eggs at 25 degrees C. Our detailed investigation has revealed that the A/Leningrad/134/17/57 (Len/17) master donor stock is a mixed population comprised of numerous variants of the ca/ts Len/17 influenza virus. We have identified these variants to exhibit a broad range in their temperature sensitive phenotype when assayed on Madin-Darby canine kidney (MDCK) cells at 37 degrees C. A selection of these variant clones has been fully characterized by sequencing in order to understand the variability in the ts phenotype. This study has also addressed the feasibility of using cell culture technology for the propagation and subsequent manufacturing of the cold-adapted influenza vaccine (CAIV), particularly with respect to retaining the defined mutations that contribute toward the ca/ts phenotype.
DOI: 10.1016/j.virusres.2004.01.026
PubMed: 15084398
Affiliations:
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Youil</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virus and Cell Biology, Merck & Co. Inc., 770 Sumneytown Pike, WP44L-206B West Point, PA 19486, USA. rima_youli@merck.com</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Virus and Cell Biology, Merck & Co. Inc., 770 Sumneytown Pike, WP44L-206B West Point, PA 19486</wicri:regionArea><wicri:noRegion>PA 19486</wicri:noRegion></affiliation></author><author><name sortKey="Kiseleva, I" sort="Kiseleva, I" uniqKey="Kiseleva I" first="I" last="Kiseleva">I. Kiseleva</name></author><author><name sortKey="Kwan, W S" sort="Kwan, W S" uniqKey="Kwan W" first="W-S" last="Kwan">W-S Kwan</name></author><author><name sortKey="Szymkowiak, C" sort="Szymkowiak, C" uniqKey="Szymkowiak C" first="C" last="Szymkowiak">C. Szymkowiak</name></author><author><name sortKey="Toner, T J" sort="Toner, T J" uniqKey="Toner T" first="T J" last="Toner">T J Toner</name></author><author><name sortKey="Su, Q" sort="Su, Q" uniqKey="Su Q" first="Q" last="Su">Q. Su</name></author><author><name sortKey="Klimov, A" sort="Klimov, A" uniqKey="Klimov A" first="A" last="Klimov">A. Klimov</name></author><author><name sortKey="Rudenko, L" sort="Rudenko, L" uniqKey="Rudenko L" first="L" last="Rudenko">L. Rudenko</name></author><author><name sortKey="Shaw, A R" sort="Shaw, A R" uniqKey="Shaw A" first="A R" last="Shaw">A R Shaw</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological</term><term>Amino Acid Substitution</term><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cold Temperature</term><term>Genetic Variation</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (growth & development)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza Vaccines</term><term>Mutation, Missense</term><term>Ovum (virology)</term><term>Phenotype</term><term>Sequence Analysis, Protein</term><term>Temperature</term><term>Vero Cells</term><term>Viral Plaque Assay</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (physiology)</term><term>Virus Cultivation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adaptation physiologique</term><term>Analyse de séquence de protéine</term><term>Animaux</term><term>Basse température</term><term>Cellules Vero</term><term>Culture virale</term><term>Lignée cellulaire</term><term>Mutation faux-sens</term><term>Méthode des plages virales</term><term>Ovule (virologie)</term><term>Phénotype</term><term>Protéines virales ()</term><term>Protéines virales (génétique)</term><term>Protéines virales (physiologie)</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Substitution d'acide aminé</term><term>Température</term><term>Vaccins antigrippaux</term><term>Variation génétique</term><term>Virus de la grippe A (croissance et développement)</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Ovule</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Ovum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Amino Acid Substitution</term><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cold Temperature</term><term>Genetic Variation</term><term>Influenza A Virus, H2N2 Subtype</term><term>Mutation, Missense</term><term>Phenotype</term><term>Sequence Analysis, Protein</term><term>Temperature</term><term>Vero Cells</term><term>Viral Plaque Assay</term><term>Virus Cultivation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Analyse de séquence de protéine</term><term>Animaux</term><term>Basse température</term><term>Cellules Vero</term><term>Culture virale</term><term>Lignée cellulaire</term><term>Mutation faux-sens</term><term>Méthode des plages virales</term><term>Phénotype</term><term>Protéines virales</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Substitution d'acide aminé</term><term>Température</term><term>Vaccins antigrippaux</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">For the past three decades the cold-adapted (ca) and temperature sensitive (ts) master donor strain, A/Leningrad/134/17/57 (H2N2) has been successfully used as the basis for the live attenuated reassortant influenza A vaccine. This donor strain was developed from A/Leningrad/134/57 (H2N2) wild-type (wt) virus following 17 passages in eggs at 25 degrees C. Our detailed investigation has revealed that the A/Leningrad/134/17/57 (Len/17) master donor stock is a mixed population comprised of numerous variants of the ca/ts Len/17 influenza virus. We have identified these variants to exhibit a broad range in their temperature sensitive phenotype when assayed on Madin-Darby canine kidney (MDCK) cells at 37 degrees C. A selection of these variant clones has been fully characterized by sequencing in order to understand the variability in the ts phenotype. This study has also addressed the feasibility of using cell culture technology for the propagation and subsequent manufacturing of the cold-adapted influenza vaccine (CAIV), particularly with respect to retaining the defined mutations that contribute toward the ca/ts phenotype.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Kiseleva, I" sort="Kiseleva, I" uniqKey="Kiseleva I" first="I" last="Kiseleva">I. Kiseleva</name><name sortKey="Klimov, A" sort="Klimov, A" uniqKey="Klimov A" first="A" last="Klimov">A. Klimov</name><name sortKey="Kwan, W S" sort="Kwan, W S" uniqKey="Kwan W" first="W-S" last="Kwan">W-S Kwan</name><name sortKey="Rudenko, L" sort="Rudenko, L" uniqKey="Rudenko L" first="L" last="Rudenko">L. Rudenko</name><name sortKey="Shaw, A R" sort="Shaw, A R" uniqKey="Shaw A" first="A R" last="Shaw">A R Shaw</name><name sortKey="Su, Q" sort="Su, Q" uniqKey="Su Q" first="Q" last="Su">Q. Su</name><name sortKey="Szymkowiak, C" sort="Szymkowiak, C" uniqKey="Szymkowiak C" first="C" last="Szymkowiak">C. Szymkowiak</name><name sortKey="Toner, T J" sort="Toner, T J" uniqKey="Toner T" first="T J" last="Toner">T J Toner</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Youil, R" sort="Youil, R" uniqKey="Youil R" first="R" last="Youil">R. Youil</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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